 1. How to install the program?

(1.1)  Install Java Runtime Environment from Sun Microsystems (JRE). The newest version of JRE can be downloaded from http://java.sun.com/javase/downloads/.


(1.2)  Create a working directory. Download the zipped file from http://www.cs.duke.edu/donaldlab/nasca.php, decompress and extract all files into the working directory.


(1.3) Install Xplor/Xplor-NIH. The newest version of Xplor/Xplor-NIH can be download from http://nmr.cit.nih.gov/xplor-nih/. 


2. How to run the program?

NASCA supports two types of interface: graphical user interface 
(GUI) and command line. To run NASCA in the GUI model, simply
 double click the executable jar file named  Nasca.jar in the 
working directory.


To run NASCA in the command line model, type the following
 command in the command window:

java -jar NascaCMD.jar -i Nasca.input -sc 
scResonancesOut.prot -noe 
noesOut.tbl

where "-i Nasca.input" specifies the input 
parameter file that gives the the paths of all input NMR files, "-sc scResonancesOut.prot" specifies the output file for
 storing the computed resonance assignments, and "-noe 
noesOut.tbl" specifies the output file for storing the computed 
NOE assignments.


 3. How to compile the program? (optional)

(3.1) Install Java Development Kit (JDK). The newest version of JRE can be downloaded from http://java.sun.com/javase/downloads/.

(3.2) Modify the Makefile under the working directory as needed. In particular, specify your working directory and the paths to your Java compiler and resources.  

(3.3) Under the working directory, type 'make'.



For more information: please visit: http://www.cs.duke.edu/donaldlab/nasca.php